Molecular Devices SpectraMax 384 Plus

The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination
for more reliable and fast data reporting with built-in cuvette port and microplate drawer.
The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard
or UV transparent microplate over a full 190 to 1000 nm wavelength range.
This 384 Plus comes from a long history of reliable tools built by Molecular Devices.
This robust system is capable of reading 96 or 384 wells, each with its own sample beam and reference detector,
for optical density measurement at a selected wavelength with endpoint (for a single point in time), kinetic (over a specific period of time),
or spectral scan mode (over a specific wavelength range).

The high-precision nine channel system (8 for the microplate; 1 for the cuvette port) consists of sample beams and reference beams that mimic a dual beam spectrophotometer.
The filter-less system includes monochromator, allowing for up to 6 wavelengths
within the visible spectrum at a time, saving time without the need to change out filters.

The PathCheck sensor eliminates optical pathlength as a factor (and corrects pipetting variation), closing the gap between microplate readers and spectrophotometers.
This is accomplished by PathCheck, in conjunction with the SoftMax Pro software, automatically normalizing well absorbance,
allowing for the elimination of standard curves, and correcting volume differences in the wells.
The 384 Plus measures liquid depth and volumes down to 5µl in each microplate well and scales the microplate data to standard 1 cm cuvette values.
The monochromator selects wavelengths in 1nm increments,
and the system is equipped with a built-in programmable linear and orbital plate shaking device.

Key applications include: DNA and RNA analysis; ELISAs and immunoassays; cell viability, proliferation, and cytotoxicity; endpoint analysis; enzyme kinetic assays; protein quantitation; endotoxin detection; and PathCheck.

INCLUDED:
The Molecular Devices SpectraMax 384 Plus Microplate Reader includes:
> Windows 10 Data Station with Pre-Loaded SoftMax Pro Software 6.5.1
> Interface Cable
> Validation Report
> Operator's Guide
> Power Cord
> Full 90 Day Parts and Labor Warranty

Specifications:
Spectral Range 190-1000 nm, tunable in 1 nm increments
Wavelength Range 190-1000 nm
Wavelength Selection Monochromator, tunable 1.0nm increments
Speed Read Mode Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds
Wavelength Bandwidth 2 nm
Wavelength Accuracy < ± 1.0 nm
Wavelength Repeatability ± 0.2 nm
Photometric Range 0.0 to 4.0 OD
Photometric Resolution 0.001 OD
Photometric Accuracy (plate) < ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Accuracy (cuvette) < ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Precision < ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Baseline Flatness < 0.001 OD
Stray Light < 0.05% @ 230 nm
Light Source Xenon Flash Lamp
Temperature Range Ambient + 4°C up to 45°C
Temperature Uniformity (plate) ± 0.5 °C @ 37°C
Dimensions 22 cm (h) x 58 cm (w) x 38 cm (d)
Weight 30lbs

Photometric Analysis Modes :
Standalone Single wavelength absorbance or %Transmittance reading of the cuvette (or test tube)

Using SoftMax Pro :
Express data as Absorbance or %Transmittance
Single wavelength reading of microplate and/or cuvette
Multiple wavelength (up to six) reading of microplate and/ or cuvette
Kinetic and kinetic graphics of microplate and/or cuvette
Spectral scan (190–1000 nm) of microplate and/or cuvette

Measurement Time (calibration off) :
Read Time (endpoint) Standard Read Microplate :
96 wells in 9 seconds (single wavelength)
96 wells in 9*N seconds (N wavelengths)
384 wells in 29 seconds (single wavelength)
384 wells in 29*N seconds (N wavelengths)

Read Time (endpoint) Speed Read Microplate:
96 wells in 5 seconds (single wavelength)
96 wells in 5*N seconds (N wavelengths)
384 wells in 16 seconds (single wavelength)
384 wells in 16*N seconds (N wavelengths)

Kinetic Read Intervals Microplate :
96 wells, 9-second minimum interval between readings (single wavelength)
1 column, 2-second minimum interval between readings (single wavelength)
384 wells, 29-second minimum interval between readings (single wavelength)

Read Time Cuvette 1 second (single wavelength)
Kinetic Read Intervals Cuvette 2-second minimum interval between readings (single wavelength)

Scan Speed:
Cuvette: Normal Scan
45*K nm/min (K = wavelength interval)
Cuvette: Speed Scan 130*K nm/min
Microplate: Normal Scan 33*K nm/min (8-well strip); 21*K nm/min (16-well strip)
Microplate: Speed Scan 135*K nm/min (8-well strip); 77*K nm/min (16-well strip)

Temperature Regulation :
Reading Chamber Isothermal when temperature regulation is not enabled
Range 4°C above ambient to 45°C when temperature regulation enabled.
The ambient temperature must be >20°C to achieve temperature regulation at 45°C.