ForteBio Octet Red96e SPR Analyzer

ForteBio’s Octet RED96e system is a multi-functional, label-free, real-time analysis instrument.
It is ideal for rapidly measuring concentration of proteins and other biomolecules, measuring kinetics and affinity.
The Octet RED96e system is a 96-well characterization of protein-protein
and protein-small molecule binding kinetics, and for the determination of protein concentrations and titer.
Popular industrial applications include expression screening, on- and off-rate screening,
epitope binning, cell line development and bioprocess optimization.

Specifications:
Detection technology: Bio-Layer Interferometry (BLI)
Biosensor type: Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking in the sensor tray
Information provided:
Yes/No binding
Kinetic and affinity analysis (kobs, ka, kd, KD)
Specific and selective detection of molecules, even in crude samples
Relative and absolute quantitation of specific proteins in crude matrices or purified samples

Data presentation:
Plots displaying real-time kinetic binding sensorgrams, fitted result plots, and residuals of fits
Concentration data analysis including calibration curves and output of tabulated concentration data
Tabulated kinetic data
Epitope binning and cross-blocking matrices and trace overlays

Sample types: Proteins, antibodies, peptides, DNA, RNA,
liposomes, bacterial cells, viruses, mammalian cells, small molecules in various media including serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates
Number of spectrometers: 8
Maximum simultaneous reads: 8
Data collection rate: 2, 5, or 10 Hz
Sample position and format: 1 standard 96-well, black, flat bottom microplate
Sample volume: 180–220 μL/well, non-destructive testing
Orbital flow capacity: Static or 100–1500 rpm
Analysis temperature range: 15–40°C, 1°C increments
Workflow: Up to 8 assays in parallel; up to 96 assays
per 96-well plate
Molecular weight detection: >150 Da
Analysis time per sample: Real-time kinetic binding experiments from 5 minutes to 4 hours, or up to 12 hours with
the evaporation cover
Association rate constant (k2): 101-107 M-1s-1
Dissociation rate constant (k2): 10-6-0.1 s-1
Affinity (KD) constant: 1 mM-10 pM
Baseline noise: ≤ 3.5 pm (RMS)
Baseline drift: ≤ 0.1 nm/hour
Workflow: Up to 8 assays in parallel; up to 96 assays per 96-well plate
Analysis time per sample: Human IgG quantitation in 2 minutes for 8 samples, ≤ 32 minutes for 96 samples
Direct quantitation range for human IgG with Protein A Biosensor: 0.05-2000 ug/mL
Dimensions (H x W x D): 19.5 in x 22 in x 18.2 in (49 cm x 56 cm x 46 cm)
Weight: 72 lb (32.7 Kg)
Electrical requirements: Mains: 100-120/200-240 VAC, 50/60 Hz, 4 A max
Power consumption: 200 W (300 W peak)
PC operating systems: Windows® 10 Professional, 64-bit, Windows 7 Professional, 64-bit, Windows 7 Professional, 32-bit
Safety standards: CE, Nemko