BioTek Synergy Neo2 Multi Mode Plate Reader

Detection mode Quad monochromators: FL, Lum., UV-Vis Abs., TRF (secondary) Filters: FL, TRF, FP, Lum., Alpha, TR-FRET, BRET
Read mode End point, kinetic, spectral scanning, well area scanning
Microplate types 1- to 1536-well plates
Other labware Compatible with Take3™ Micro-Volume Plates with 2 µL
Temperature control To 65 ºC with Condensation Control
Variation ±0.2 ºC at 37 ºC
Shaking Linear, orbital, double orbital
Software Gen5 Microplate Reader and Imager Software included

Gen5 Secure Software option for 21 CFR Part 11 compliance features
Automation BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible
Barcode reader Multi-directional, 1D and 2D camera-based
Read height Auto Z, 0.1 mm steps, top/bottom (Filters), top (Mono)
Kinetic speed 96-well: 6 seconds; 384-well: 11 seconds; 1536-well: 25 seconds
With Neo Stacker minimum processing time per plate :
96-well: 20 seconds
384-well: 25 seconds
1536-well: 39 seconds

Absorbance
Light source Xenon flash lamp
Wavelength selection Monochromator
Wavelength range 230 – 999 nm, 1 nm increment
Bandwidth 2 nm (230 – 285 nm), 4 nm (>285 nm)
Dynamic range 0 – 4.0 OD
Resolution 0.0001 OD
Fluorescence Intensity
Sensitivity: Filter Cubes :
Fluorescein 0.2 pM (384-well low volume plate) – Top
Fluorescein 1 pM (1536-well plate) – Top
Fluorescein 1 pM (384-well plate) – Bottom

Quadruple Monochromator :
Fluorescein 2 pM (384-well low volume plate) – Top
Fluorescein 2.5 pM (384-well plate) – Bottom
Light source High energy xenon flash lamp
Read height Auto Z, 0.1 mm steps, top/bottom (Filters), top (Mono)
Wavelength selection Double grating monochromators (top/bottom)

Filters (top/bottom)
Wavelength range Monochromators: 250 – 850 nm
Filters Cubes 200 – 850 nm
Monochromator
Bandwidth Variable, from 3 nm to 50 nm in 1 nm increment excitation/emission
Detection system :
Single PMT or dual PMTs (top filter system)
Low Noise PMT bottom filter system
Red shifted PMT top/bottom monochromator system

Luminescence
Sensitivity 5 amol ATP (flash) (384-well low volume plate)
Wavelength range 300 – 700 nm
Dynamic range >6 decades
Fluorescence Polarization
Sensitivity 1 mP standard deviation at 1 nM fluorescein (384-well low volume plate) 1.5 mP standard deviation at 1 nM fluorescein (1536-well plate)
Light source Xenon flash lamp
Wavelength selection Filter cubes (top/bottom)
Wavelength range 280 – 850 nm
Detection system Single PMT or dual PMTs
Time-Resolved Fluorescence
Light source Xenon flash lamp
Sensitivity :
Europium 40 fM (384-well low volume plate)
Europium 70 fM (1536-well plate)
Wavelength selection Filter Cubes (top/bottom) Double grating monochromator (top/bottom)
Wavelength range Monochromators: 250 – 850 nm
Filters 200 – 850 nm
Detection system Single PMT or dual PMTs

Alpha
Sensitivity 100 amol LCK peptide (384-well low volume plate)
Light source 100 mW 680 nm laser
Wavelength selection Filter cubes
Read speed :
96-well: 30 seconds
384-well: 1 minute 50 seconds
1536-well: 7 minutes 20 seconds
Dimension :
20x15x16- Plate Reader
18x7x27- Biostacker
Weight 80lbs
Power 120V Power Cords (12A), 250 Watts max