Attune NxT Flow Cytometer

Specifications:
Optical alignment Fixed alignment with pre-aligned welded fiber; no user maintenance required
Laser Features : Blue Excitation Laser (488 nm), Red Laser (637 nm)
Number of detection channels installed 9 (4 blue, 3 red, forward and side scatter)
Number of detection channels capable Up to 16
Number of lasers capable Up to 4
Number of detection channels possible 2 scatter channels, up to 14 fluorescence channels
Sample flow rates (µL/min) 12.5–1,000
Fluorescence sensitivity ≤80 MESF for FITC, ≤30 MESF for PE, ≤70 MESF for APC
Fluorescence resolution CV
Maximum electronic speed Up to 35,000 events/sec with all parameters
Instrument tracking Automated daily baseline and performance test with Levey-Jennings plots
Format Tubes
Autosampler :
Compatible plate types 96 deep-well (flat, round, and V-bottom) 96-well standard depth (flat, round, and V-bottom)
384-well standard depth (flat, round, and V-bottom)
384 deep-well (flat, round, and V-bottom)
Minimum dead volume (single draw) :
30 L for 12.5200 L/min
50 L for 501,000 L/min
Wash cycle User-defined number of wash cycles, dependent on plate-processing protocol and time to acquire plates
Mixing method Each well mixed via full aspiration (no shaking)
Sample mixing Mixing optimized to preserve cell viability; mixing cycles optimized to sample analysis volume
Performance High-throughput mode acquisition time: