Applied Biosystems StepOne Real-Time qPCR System

Format: 48-well Plate, 0.1 ml Tubes
Thermal Accuracy: 0.25°C (35°C to 95°C) of Display Temperature
Sample Ramp Rate: Fast Mode: ± 2.2°C⁄s, Standard mode: ± 1.6°C⁄s
Sensitivity: 1 Copy
Temperature Range (Metric): 4 to 100°C
Reaction Volume Range: 10-30 μl (Standard Curve Experiments: 40 μl in Standard Mode is Validated)
Passive Reference Dye: ROX (Separate Tube), No ROX, ROX (Pre-mixed)
Dynamic Range: Linear Dynamic Range greater than 9 Log Units (Detection)
Calibrated Dye: VIC™, SYBR™ Green I, JOE™, FAM™, ROX™
High-throughput Compatibility: Multiplexing
For Use With (Equipment): StepOne
Thermal Uniformity: 0.25°C (35°C to 95°C) of Setpoint/Display Temperature
Reaction Speed: Fast, Standard
Detection Method: SYBR, Primer-Probe Detection
Run Time: