Applied Biosystems ABI 7300 Real-Time PCR System

Thermal cycling system: Peltier-based, 96-well block
Sample Ramp Rate: +/-1.1°C/sec
Peak Block Ramp Rate: 1.6°C/sec
Temperature Range: 4°C – 100°C
Temperature Accuracy: ±0.25°C of setpoint/display temperature measured at 3 minutes after clock start
Temperature Uniformity: ±0.50°C, 30 seconds after clock start
Optical system : Single excitation, four emission filters, and CCD camera
Passive Reference Dyes: ROX or any calibrated dye.
Data Collection: Data collected in all 4 filters for all wells regardless of plate setup. Plate setup may be altered after run completes.
Quantitative PCR run time: < 1 hour 50 minutes
Supported Volumes: 20 – 100 μL